Method for detecting resistance to mosaic disease of tobacco germplasm by specific primer

• Main Authors: PAN DAREN, ZHOU YIFEI, SHAN RUIYANG, FANG PAN, ZHANG YANYUN, GUO JINPING
• Format: Patent
• Language: Chinese; English
• Published: 05.10.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a method for detecting the resistance characteristic of tobacco germplasm to a mosaic disease by a specific primer. The nucleotide sequence of the specific primer is: CNF: 5'-AGGAAGGAAAGCACCAAATGG-3'; CNR: 5'-CGCAACCCGTATTCAACTCC-3'. The genome DNA of tobacco germplasm to be detected is processed by PCR amplification with the specific primer; if a 565bp product is specifically amplified, the resistance can be determined. The invention meets the requirements of detecting the resistance characteristic to the mosaic disease of tobacco germplasm at a molecular level, can prevent the viral pollution easily caused by traditional field detection methods, and has the characteristics of simple operations and the like.