Multiple RT-PCR kit for enterovirus
The invention discloses a multiple reverse transcription-polymerase chain reaction (RT-PCR) kit for enterovirus, and relates to a PCR kit which belongs to the field of biotechnology. The kit comprises Moloney murine leukemia virus (M-MLV) reverse transcriptase, a ribonucleic acid (RNA) enzyme inhibi...
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Format | Patent |
Language | Chinese English |
Published |
14.09.2011
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Subjects | |
Online Access | Get full text |
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Abstract | The invention discloses a multiple reverse transcription-polymerase chain reaction (RT-PCR) kit for enterovirus, and relates to a PCR kit which belongs to the field of biotechnology. The kit comprises Moloney murine leukemia virus (M-MLV) reverse transcriptase, a ribonucleic acid (RNA) enzyme inhibitor, 5xRT-PCR Buffer, a positive control, a negative control, a random primer, three pairs of specific primers, multiple 10xPCR Buffer and thermus aquaticus (Taq) enzyme. In the kit, universal primers F1 and F2, namely 5'-ACAAGCACTTCTGTTTCCCCGG-3' and 5'-GATTGTCACCATAAGCAGCCA-3', are designed according to a non-coding area at the 5' end of a conserved sequence of an enterovirus gene; a pair of primers is designed according to a conserved sequence of a gene VP1 of virus EV71; and a pair of primers is designed according to a conserved sequence of the gene VP1 of virus CA16. The kit is short in period of detection time, high in detection efficiency, specificity of virus detection and accuracy, can perform the qualitat |
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AbstractList | The invention discloses a multiple reverse transcription-polymerase chain reaction (RT-PCR) kit for enterovirus, and relates to a PCR kit which belongs to the field of biotechnology. The kit comprises Moloney murine leukemia virus (M-MLV) reverse transcriptase, a ribonucleic acid (RNA) enzyme inhibitor, 5xRT-PCR Buffer, a positive control, a negative control, a random primer, three pairs of specific primers, multiple 10xPCR Buffer and thermus aquaticus (Taq) enzyme. In the kit, universal primers F1 and F2, namely 5'-ACAAGCACTTCTGTTTCCCCGG-3' and 5'-GATTGTCACCATAAGCAGCCA-3', are designed according to a non-coding area at the 5' end of a conserved sequence of an enterovirus gene; a pair of primers is designed according to a conserved sequence of a gene VP1 of virus EV71; and a pair of primers is designed according to a conserved sequence of the gene VP1 of virus CA16. The kit is short in period of detection time, high in detection efficiency, specificity of virus detection and accuracy, can perform the qualitat |
Author | SHI KANG WANG YAN TANG XINGCHUN XIONG YING XIANG RONG ZHANG QI AI HONGWU LIU YINGLE HU XIAOJING WU JIANGUO CHENG YIFENG LI TONGYA LIU WEIYONG |
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Snippet | The invention discloses a multiple reverse transcription-polymerase chain reaction (RT-PCR) kit for enterovirus, and relates to a PCR kit which belongs to the... |
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Title | Multiple RT-PCR kit for enterovirus |
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