Multiple RT-PCR kit for enterovirus

• Main Authors: XIANG RONG, ZHANG QI, LI TONGYA, LIU YINGLE, XIONG YING, TANG XINGCHUN, HU XIAOJING, WU JIANGUO, AI HONGWU, CHENG YIFENG, SHI KANG, LIU WEIYONG, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 14.09.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a multiple reverse transcription-polymerase chain reaction (RT-PCR) kit for enterovirus, and relates to a PCR kit which belongs to the field of biotechnology. The kit comprises Moloney murine leukemia virus (M-MLV) reverse transcriptase, a ribonucleic acid (RNA) enzyme inhibitor, 5xRT-PCR Buffer, a positive control, a negative control, a random primer, three pairs of specific primers, multiple 10xPCR Buffer and thermus aquaticus (Taq) enzyme. In the kit, universal primers F1 and F2, namely 5'-ACAAGCACTTCTGTTTCCCCGG-3' and 5'-GATTGTCACCATAAGCAGCCA-3', are designed according to a non-coding area at the 5' end of a conserved sequence of an enterovirus gene; a pair of primers is designed according to a conserved sequence of a gene VP1 of virus EV71; and a pair of primers is designed according to a conserved sequence of the gene VP1 of virus CA16. The kit is short in period of detection time, high in detection efficiency, specificity of virus detection and accuracy, can perform the qualitat