Quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis

• Main Authors: CHEN BISHENG, QIN JUN, GAO YAN, CAI XINFENG, YIN LIQING, ZHAO XIAOFENG, ZHU HUAFANG, LI JIAN, LIU ZHAO, HU YONGHONG, ZHANG YALI
• Format: Patent
• Language: Chinese; English
• Published: 14.09.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; FISHING; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING; WATERING

The invention discloses a quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis, which comprises the following steps of the selection and disinfection of explants, induction culture, multiplication culture, root induction culture and transplantation rooting. In the tissue culture propagation method for the narcissus tazetta var. chinensis, the complete technical process from the selection of the explants to the transplantation of the explants is accomplished, and bulbs subjected to the multiplication culture are rooted and induced within short time and are transplanted to a perlite substrate for rooting directly, so that test tube seedlings of the narcissus tazetta var. chinensis are not needed to be rooted in vitro; and the steps of induction rooting and transplantation are simplified, the production period is shortened, the cost is reduced, and the survival rate of the transplantation reaches over 92 percent. The method is high in pertinence, simple and easy and high