Method for detecting surface enhanced Raman spectra of blood RNA

• Main Authors: FENG SHANGYUAN, XI GANGQIN, LIN XIAOXI, CHEN XIWEN, CHEN YANPING, CHEN RONG, LIN DUO
• Format: Patent
• Language: Chinese; English
• Published: 07.09.2011
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a preparation method of a silver nanometer membrane for detecting surface enhanced Raman spectra (SERS) of ribonucleic acid (RNA), and belongs to the technical field of medical science. In the method, the RNA in blood is extracted, and SERS spectra with strong signals and more spectrum peaks are acquired by taking the prepared silver nanometer membrane as an enhanced substrate to reflect the information of RNA molecules fully; and the enhanced substrate prepared by the method is contrasted to various enhanced substrates simultaneously to obtain the conclusion that the information acquired in the process of detecting the RNA molecules by using the enhanced substrate is the most and highest in strength, so high-quality SERS spectrograms can be acquired. Therefore, the high-quality spectrograms can be used for statistic analysis to obtain differences between patients and normal person so as to establish the foundation for the application of SERS detection to clinical diagnosis. The metho