Method for analyzing result of loop-mediated isothermal amplification

• Main Authors: MA SHAOSAI, ZHANG YAN, CUI ZHENGGUO, CHEN BIJUAN, LI QIUFEN, ZHANG XUZHI
• Format: Patent
• Language: Chinese; English
• Published: 03.08.2011
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for analyzing a result of loop-mediated isothermal amplification (LAMP), aiming at establishing a new method for analyzing the result of LAMP reaction, in which qualitative and quantitative information of a target gene is obtained by determining the change of electric conductivity of an LAMP solution; the method comprises the working steps: (1) thin wall tubes are inserted into a working sensor of an inductive conductivity gauge to serve as LAMP reaction tanks; (2) the value of electric conductivity of mixed solution before and after the biochemical reaction in every reaction tank is determined on line; and (3) the result of LAMP reaction can be deduced from the change of electric conductivity of each reaction unit so as to represent target DNA information. The method has the characteristics of being simple, convenient and fast, high in sensitivity, simple and convenient in operation, simple and inexpensive in instrument and equipment and low in expenditure, and can realize b