Real-time fluorescence quantitative PCR (polymerase chain reaction) kit for herpes simplex virus

• Main Authors: XIANG RONG, ZHANG QI, LI TONGYA, LIU YINGLE, YANG CAO, ZOU MENGYUE, XIONG YING, CHEN SHA, SHI QING, WANG SHAN, WU JIANGUO, AI HONGWU, CHENG YIFENG, SHI KANG, LIU WEIYONG
• Format: Patent
• Language: Chinese; English
• Published: 03.08.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a real-time fluorescence quantitative PCR (polymerase chain reaction) kit for herpes simplex virus, relating to the PCR kits in the biotechnology field. The kit comprises a pair of specific primers, a specific fluorescent probe, positive control, negative control, 5*PCR buffer and Enzyme Mix of the herpes simplex virus, wherein the designed specific primers are F:5'-ACTCGAGTGCGAAAAGACGTTCT-3' and R:5'-GTATCGTCGTAAAACAGCAGGTC-3'; and the designed probe is FP: FAM-AAAGGGCGTGGATCTGGTGCGT-BHQ-1. The kit has the following advantages and positive effects: the detection time period is short and the detection efficiency is high; the specificity and accuracy rate in detecting the virus are high; quantitative analysis of the virus can be carried out while carrying out qualitative analysis of the virus; the detection sensitivity is higher than the sensitivity in the common PCR and immunological detection methods; the kit is easy to operate and popularize; and the experimental results have good re