Dog B lymphocyte activating factor cDNA, cloning method and recombination application thereof

• Main Authors: ZHANG SHUANGQUAN, SHEN YUEFEN, WANG SHULE
• Format: Patent
• Language: Chinese; English
• Published: 13.07.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a dog B lymphocyte activating factor (dog BAFF) cDNA, a cloning method and recombination application thereof, relating to the field of biological genetic engineering. A gene of the dog B lymphocyte activating factor has a sequence shown in SEQ ID NO.1. The cloning method comprises the steps of: extracting total dog spleen RNA by using a TRIzol method and carrying out reverse transcription; analyzing human, rat, chick, duck and goose BAFFcDNA conversed regions through Clustalw software, and designing a degenerate primer for carrying out PCR (Polymerase Chain Reaction) amplification to obtain a 340bp amplification segment P; and designing a specific primer according to the 340bp amplification segment P and cloning a full-length BAFFcDNA through RACEPCR. The dog B lymphocyte activating factor cDNA can be used for producing a recombinant dog BAFF as an immune potentiator for canoidea through the traditional genetic engineering method.