Gold nanoparticle template for promoting cell growth and preparation method thereof

• Main Authors: RONG HUILIN, LI JINRU, LU WENSHENG, ZHANG YAKUN, JIANG LONG, CUI WENJUAN, LIN GUANHUA
• Format: Patent
• Language: Chinese; English
• Published: 06.07.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a gold nanoparticle template for promoting cell growth and a preparation method thereof. The preparation method of the gold nanoparticle template comprises the following the steps of: printing a micro-nano gold array pattern on a substrate by using ink-jet water-soluble ink which contains gold nanoparticles through an ink-jet printer; and performing calcination or laser etching on the substrate with the array pattern and removing a high polymer protective agent on the surfaces of the gold nanoparticles to obtain the gold nanoparticle template. A preparation method of the ink-jet water-soluble ink which contains the gold nanoparticles comprises the following steps of: ultrasonically mixing the gold nanoparticles, a water-soluble cosolvent, a high polymer dispersing agent, a surfactant and secondary distilled water; and pre-dispersing and concentrating to obtain the ink-jet water-soluble ink. The gold nanoparticle template can be co-cultured with cells to obtain a cell biological chip. B