Biochip and preparation method thereof

• Main Authors: RONG HUILIN, LI JINRU, LU WENSHENG, ZHANG YAKUN, JIANG LONG, CUI WENJUAN, LIN GUANHUA
• Format: Patent
• Language: Chinese; English
• Published: 01.06.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a biochip and a preparation method thereof. The preparation method of the biochip comprises the following steps of: printing a micro-nano metal array pattern on a substrate by using metal nanoparticle-containing ink-jet water-soluble ink through an ink jet printer; curing, performing sterilization and disinfection treatment on the substrate with the array pattern, and then co-culturing the substrate with a biological material to obtain the biochip, wherein a preparation method of the jet-ink water-soluble ink comprises the following steps of: mixing metal nanoparticles, a water-soluble cosolvent, a high-molecular dispersing agent, a surfactant and secondary distilled water with stirring; pre-dispersing; and concentrating to obtain the ink. The chip prepared by the method is lower in cost; a preparation process is simple, and is easy to master and convenient to popularize; the biochip is quickly detected and is environmentally-friendly; and compared with similar products at home and abr