Method for screening effective shRNA of triacylglycerol hydrolase gene

• Main Authors: WANG WEI, ZHAO WANGSHENG, LUO JUN, TENG YANLING
• Format: Patent
• Language: Chinese; English
• Published: 20.04.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1, designing and synthesizing a single-chain DNA oligonucleotide segment for coded target shRNA; A2, constructing a pENTR/CMV-GFP/U6-shRNA carrier;...