Method for screening effective shRNA of triacylglycerol hydrolase gene

The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1, designing and synthesizing a single-chain DNA oligonucleotide segment for coded target shRNA; A2, constructing a pENTR/CMV-GFP/U6-shRNA carrier;...

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Bibliographic Details
Main Authors WANG WEI, ZHAO WANGSHENG, LUO JUN, TENG YANLING
Format Patent
LanguageChinese
English
Published 20.04.2011
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1, designing and synthesizing a single-chain DNA oligonucleotide segment for coded target shRNA; A2, constructing a pENTR/CMV-GFP/U6-shRNA carrier; A3, constructing a pDsRed1-C1-ATGL carrier; and A4, screening an effective sequence of the shRNA. Because an interference vector and a target gene are marked by red fluorescence and green fluorescence respectively, the process of screening interference sequences is time-saving and high in efficiency, the result is more intuitive and reliable, and a plurality of sequences can be screened at the same time.
Bibliography:Application Number: CN20101535004