Method for screening effective shRNA of triacylglycerol hydrolase gene

The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1, designing and synthesizing a single-chain DNA oligonucleotide segment for coded target shRNA; A2, constructing a pENTR/CMV-GFP/U6-shRNA carrier;...

Full description

Saved in:
Bibliographic Details
Main Authors WANG WEI, ZHAO WANGSHENG, LUO JUN, TENG YANLING
Format Patent
LanguageChinese
English
Published 20.04.2011
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

Abstract The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1, designing and synthesizing a single-chain DNA oligonucleotide segment for coded target shRNA; A2, constructing a pENTR/CMV-GFP/U6-shRNA carrier; A3, constructing a pDsRed1-C1-ATGL carrier; and A4, screening an effective sequence of the shRNA. Because an interference vector and a target gene are marked by red fluorescence and green fluorescence respectively, the process of screening interference sequences is time-saving and high in efficiency, the result is more intuitive and reliable, and a plurality of sequences can be screened at the same time.
AbstractList The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1, designing and synthesizing a single-chain DNA oligonucleotide segment for coded target shRNA; A2, constructing a pENTR/CMV-GFP/U6-shRNA carrier; A3, constructing a pDsRed1-C1-ATGL carrier; and A4, screening an effective sequence of the shRNA. Because an interference vector and a target gene are marked by red fluorescence and green fluorescence respectively, the process of screening interference sequences is time-saving and high in efficiency, the result is more intuitive and reliable, and a plurality of sequences can be screened at the same time.
Author WANG WEI
TENG YANLING
LUO JUN
ZHAO WANGSHENG
Author_xml – fullname: WANG WEI
– fullname: ZHAO WANGSHENG
– fullname: LUO JUN
– fullname: TENG YANLING
BookMark eNqNyr0KwjAUBtAMOvj3DtcHEJI6OZZicbGDuJeQfmkCIbckQcjb28EHcDrL2YtN5Iid6J8ojieynCibBEQfZ4K1MMV_QNm9hpbYUklemxrmUA0SB3J1WtEZNCPiKLZWh4zTz4M49_d397hg4RF50WZNZewGJRvZKHWT7fWf8wVE6zU2
ContentType Patent
DBID EVB
DatabaseName esp@cenet
DatabaseTitleList
Database_xml – sequence: 1
  dbid: EVB
  name: esp@cenet
  url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP
  sourceTypes: Open Access Repository
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Chemistry
Sciences
ExternalDocumentID CN102021190A
GroupedDBID EVB
ID FETCH-epo_espacenet_CN102021190A3
IEDL.DBID EVB
IngestDate Fri Jul 19 12:41:40 EDT 2024
IsOpenAccess true
IsPeerReviewed false
IsScholarly false
Language Chinese
English
LinkModel DirectLink
MergedId FETCHMERGED-epo_espacenet_CN102021190A3
Notes Application Number: CN20101535004
OpenAccessLink https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110420&DB=EPODOC&CC=CN&NR=102021190A
ParticipantIDs epo_espacenet_CN102021190A
PublicationCentury 2000
PublicationDate 20110420
PublicationDateYYYYMMDD 2011-04-20
PublicationDate_xml – month: 04
  year: 2011
  text: 20110420
  day: 20
PublicationDecade 2010
PublicationYear 2011
RelatedCompanies NORTHWEST A&F UNIVERSITY
RelatedCompanies_xml – name: NORTHWEST A&F UNIVERSITY
Score 2.9141285
Snippet The invention discloses a method for screening effective shRNA of adipose triacylglycerol hydrolase gene. The method comprises the following steps of: A1,...
SourceID epo
SourceType Open Access Repository
SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Title Method for screening effective shRNA of triacylglycerol hydrolase gene
URI https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110420&DB=EPODOC&locale=&CC=CN&NR=102021190A
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fS8MwED7mFPVNp6LzBxGkb8V1pt36MGRLW4awbowpextNkziltGOrSP3rvXar80WfEhIISch33-VyuQO4MxQPhKWEHjS50qmpTN3mQahzW-X00mjJtZevb_Wf6dPUnFbgvfwLU8QJ_SyCIyKiQsR7WsjrxdaI5RS-lat7_oZNyaM36TiaKM19eAYbmtPruKOhM2QaYx3ma_4YdV285RvIft0d2EU1upWjwX3p5b9SFr8pxTuCvRGOFqfHUPma1-CAlZnXarA_2Dx4Y3WDvdUJeIMi2TNBLZMg1vH-iaxD1v4YKLLIaj72uyRRJE_EEWbRa5SFcplEZJ4JLJCtCB4WeQq3njthfR0nNPtZ_Yz527k_nEE1TmJ5DkSE1BKGFJyrAEnYbjeNgFLJVVtQgVLsAup_j1P_r_MSDteGU4oQuoJquvyQ18i8Kb8ptuwb7ZuJYg
link.rule.ids 230,309,783,888,25576,76882
linkProvider European Patent Office
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fT8IwEL4gGvFNUaP4qyZmb4sMCo4HYqBjQWWDEDS8kXVtRUMYYTNm_vXeNhBf9KlNmzRt0---6_V6B3BjKO6JuhK6V-FKpzVV0xvc83XeUAm9lO9k5uXr1rvP9HFcG-fgff0XJo0T-pkGR0RE-Yj3KJXXi40Ry0p9K8Nb_oZNwb09alqaWJv78AyWNavd7Az6Vp9pjDWZq7lD1HXxlm8g-7W2YBtVbDPJdtB5aSe_Uha_KcXeh50BjjaPDiD3NS1Cga0zrxVh11k9eGN1hb3wEGwnTfZMUMskiHW8fyLrkMwfA0UWCadDt0UCRZJEHH48e53FvlwGMzKNBRbIVgQPizyCa7szYl0dJzT5Wf2EuZu5V48hPw_m8gSI8GldGFJwrjwk4YZZMTxKJVemoAKl2CmU_h6n9F_nFRS6I6c36T24T2ewlxlRKcLpHPLR8kNeIAtH_DLdvm-HbIxS
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Method+for+screening+effective+shRNA+of+triacylglycerol+hydrolase+gene&rft.inventor=WANG+WEI&rft.inventor=ZHAO+WANGSHENG&rft.inventor=LUO+JUN&rft.inventor=TENG+YANLING&rft.date=2011-04-20&rft.externalDBID=A&rft.externalDocID=CN102021190A