Liquid fermentation method for producing salt-tolerant cellulase from sea aspergillus niger

• Main Authors: YAO SHANJING, LIU JIEFENG, LIN DONGQIANG, XUE DONGSHENG
• Format: Patent
• Language: Chinese; English
• Published: 13.04.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a liquid fermentation method for producing salt-tolerant cellulase from sea aspergillus niger. The liquid fermentation method comprises the following steps: 1) screening soil in 10-20m depth of coastal area of the East China Sea to obtain a strain of sea aspergillus niger; 2) inoculating the sea aspergillus niger into a PDA (potato dextrose agar) culture medium, culturing at 32-40 DEG C for 60-90 hours to obtain slant spores, and preserving the slant spores at 4 DEG C; 3) inoculating the slant spores on a PDA-1 culture medium, and culturing at 32-40 DEG C for 60-90 hours to obtain activated spores; 4) inoculating the activated spores on a slant of an eggplant bottle, and culturing at 42 DEG C for 74 hours to obtain eggplant bottle slant spores subject to expanding culture; and 5) utilizing sterile artificial sea water to dilute the eggplant bottle slant spores obtained by expanding culture to 108 spores/ml, inoculating on a liquid fermentation enzyme productionculture medium, and ferme