Method for purifying effective ingredients of cicer ariethnum L. bean sprouts

• Main Authors: AJIAIKEBAIER.AISA, LV QIAOYING, DOU JUN, ABULIMITI.YILI, GAO YANHUA, CHENG ZHEN
• Format: Patent
• Language: Chinese; English
• Published: 06.04.2011
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention relates to a method for purifying effective ingredients of cicer ariethnum L. bean sprouts. The method comprises the following steps of: selecting superior cicer ariethnum L.; germinating; picking the bean sprouts; drying; crushing; adding alcohol; heating for refluxing and extracting; collecting the extracting solution; recycling the alcohol under reduced pressure to obtain the extracting solution; then purifying with polyamide which is a macroporous resin; eluting with alcohol solution; concentrating under reduced pressure; and drying in vacuum to obtain the effective ingredients of the cicer ariethnum L. bean sprouts, such as total saponins and total isoflavones. The effective ingredients of the cicer ariethnum L. bean sprouts such as total saponins and total isoflavones proved by an in vitro sugar reducing experiment have inhibition effect to protein tyrosine phosphatase 1B and have good activity. The content of the effective ingredients of the cicer ariethnum L. bean sprouts, such as total