Paper-based cellular arrays

• Main Authors: LAROMAINE SAGUE ANNA, WHITESIDES GEORGE M, DERDA RATMIR
• Format: Patent
• Language: Chinese; English
• Published: 16.02.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

Three-dimensional cellular arrays, methods of making three-dimensional cellular arrays, and methods of identifying agents using the arrays are disclosed. A three-dimensional cellular array includes a porous, hydrophilic substrate comprising a plurality of porous regions, each porous region bounded at least in part by a liquid impervious boundary; and a hydrogel comprising cells, wherein the hydrogel is embedded within the porous regions. A method of making a three-dimensional cellular array comprises providing a porous, hydrophilic substrate, wherein the substrate comprises a plurality of porous regions, each porous region bounded at least in part by a liquid impervious boundary; and contacting the porous, hydrophilic substrate with a suspension of cells and a temperature-sensitive hydrogel or an ionotropic hydrogel precursor, wherein the suspension saturates one or more porous regions of the substrate. A method of identifying an agent comprises providing an array described that modifies cellular function; co