Human FGF21 mutant gene and method for preparing recombinant human FGF21 protein

The invention discloses a human FGF21 mutant gene and a method for preparing a recombinant human FGF21 protein. The mutation is carried out on a human FGF21 gene, the base sequence after the mutation is as shown in SEQ IDNO.1, and the expression level of the gene after the mutation in prokaryocytes...

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Bibliographic Details
Main Authors REN GUIPING, LI DESHAN, GAO HUASHAN, LIU MINGYAO, WANG WENFEI
Format Patent
LanguageChinese
English
Published 09.02.2011
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a human FGF21 mutant gene and a method for preparing a recombinant human FGF21 protein. The mutation is carried out on a human FGF21 gene, the base sequence after the mutation is as shown in SEQ IDNO.1, and the expression level of the gene after the mutation in prokaryocytes is significantly improved. The invention further provides a method for improving the expression level of the human FGF21 protein, which comprises the following steps: sequentially connecting a 6 multiplied by His tag gene, an SUMO tag gene, a hydroxylamine cleavage site gene and the human FGF21 mutant gene together, and then obtaining a gene as shown in SEQ ID NO.3; connecting the obtained gene with an expression vector, and obtaining a recombinant expression vector; transforming into host cells; culturing, inducing the expression of the recombinant protein, collecting and purifying the expressed protein, carrying out hydroxylamine cleavage, and further purifying. The method has the advantages of good specificity,
Bibliography:Application Number: CN20101274258