Preparation method for natural product podophyllotoxin

• Main Author: SU LIUHUA
• Format: Patent
• Language: Chinese; English
• Published: 09.02.2011
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HETEROCYCLIC COMPOUNDS; METALLURGY; ORGANIC CHEMISTRY; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE

The invention relates to a preparation method for natural product podophyllotoxin, which comprises the following steps of pulverizing raw materials into 20 to 60 meshes; taking CO2 and chloroform as solvents; super-critically extracting for 1 to 3 hours; washing analytic matters by utilizing deionized water; dissolving by utilizing chloroform; carrying out a medium-pressure alumina column chromatography; carrying out gradient elution by utilizing a petroleum ether-methanol chloroform mixing solution; collecting high-concentration fraction, concentrating and crystallizing; and recrystallizing the methanol chloroform mixing solution. The method for producing the podophyllotoxin has the advantages of less pollution, high extraction efficiency and less usage of organic solvents.