DNA (Deoxyribonucleic Acid) molecule for encoding pig alpha-interferon and recombinant colibacillus as well as application thereof

• Main Authors: CUI ZENGXUE, SHI QIAO, LIU HUANZHEN, MA FENGLONG, LI CHUNRONG
• Format: Patent
• Language: Chinese; English
• Published: 26.01.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a DNA (Deoxyribonucleic Acid) molecule for encoding pig alpha-interferon and recombinant colibacillus as well as application thereof. A nucleotide sequence of the DNA molecule for encoding the pig alpha-interferon is from 5' end 1st to 501st of a sequence 1 in a sequence table. A recombinant bacterium for expressing the pig alpha-interferon is the recombinant colibacillus for stably expressing a protein encoded by the DNA molecule shown by 5' end 1st to 498th of the sequence 1 in the sequence table. The invention also discloses a method for producing a pig alpha-interferon protein by utilizing the recombinant bacterium for expressing the pig alpha-interferon. The DNA molecule for encoding the pig alpha-interferon is suitable for expression in the colibacillus. The pig alpha-interferon is safe to a pig, without adverse influences on piglet daily gain, growing pig feed to gain ratio and pregnant sow productivity and reproductive performance, and has obvious treating effect to diagnosed c