Method of preparing lactalbumin scale purification

• Main Authors: ZHAI DONGGAI, HUO CHENXI, WANG YI, LI XUE, LI RONGXIU
• Format: Patent
• Language: Chinese; English
• Published: 19.01.2011
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention provides a method of preparing lactalbumin scale purification in the technical field of biomedical, comprising the following steps: synthesizing affinity separation material with special milk Beta-lactoglobulin; implementing derivatization to the basic chromatographic medium through the basic chromatographic medium with amino; reacting and activating the basic chromatographic medium with amino with trichlorine triazine razine, and then reacting with triethylene tetramine to synthesis the afinity separation material, so as to obtain Alpha-serum albumin purity; purifying the Beta-lactoglobulin through the prepared afinity separation material; after heating the serum, purifying the Alpha-serum albumin through the prepared afinity separation material. The method overcomes the defect in the purification technique of the Alpha-serum albumin, reduces the steps of separation purification in the production of the Alpha-serum albumin, reduces the production cost and improves the producing efficiency. The