Brassica napus mutator gene for resisting imidazolone weedicide and application thereof

• Main Authors: ZHANG JIEFU, CHEN XINJUN, PU HUIMING, HU MAOLONG, GU HUI, CHEN SONG, LONG WEIHUA, CHEN FENG, GAO JIANQIN, QI CUNKOU
• Format: Patent
• Language: Chinese; English
• Published: 05.01.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WATERING; WINE

The invention discloses a brassica napus mutator gene for resisting imidazolone weedicide and application thereof, relating to the field of plant molecule breeding. Primer pairs are utilized to carry out PCR (Polymerase Chain Reaction) proliferation by using a mutant M9 of brassica napus for resisting imidazolone weedicide as a material to obtain a mutator gene of the mutant with the sequence number of SEQ ID NO.1; and proteins are acquired by translation of the gene with an amino acid sequence of SEQ ID No.2. By applying the conventional breeding method of crossbreeding plants, backcrossing plants and the like, the gene is introduced into other napus varieties or variety series having no resistance to the imidazolone weedicide, so as to improve the durability on the imidazolone weedicide by introducing ALS gene mutation nucleotide sequence target varieties or variety series.