Real-time fluorescent RCR molecular detection kit for leptosphaeria maculans and detection method thereof

The invention relates to a real-time fluorescent RCR molecular detection kit for leptosphaeria maculans, which contains a general hybrid reagent for gene expression, ddH2O, a probe and primer mixture and a positive control. The probe and primer mixture contains 5mumol/L of LMpro probe, 18mumol/L of...

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Bibliographic Details
Main Authors AN YULIN, WU XINHUA, SU HAN, SUN MINQIN, CHEN GUANYUAN, LI BIN, WU JING, WU CUIPING
Format Patent
LanguageChinese
English
Published 14.11.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a real-time fluorescent RCR molecular detection kit for leptosphaeria maculans, which contains a general hybrid reagent for gene expression, ddH2O, a probe and primer mixture and a positive control. The probe and primer mixture contains 5mumol/L of LMpro probe, 18mumol/L of LMf upstream primer and 18mumol/L of LMr downstream primer; and the positive control is 100ng/muL leptosphaeria maculans DNA solution. The kit can directly detect the sick rape seed or plant residues, has the advantages of quickness, sensitivity, accuracy and good repeatability, particularly can beused for detecting a sample with low pathogenic bacteria content, is particularly suitable for quickly detecting the leptosphaeria maculans in seeds, plants and sick residues of cruciferous crops such as imported and exported rape seeds and the like, and has good application prospect.
Bibliography:Application Number: CN201019026063