Tri-antibody sandwich ELISA detection method of IgG of tree shrew

• Main Authors: FENG YUE, WEI DAQIAO, WEN ZHIGANG, YANG HENG, XIA XUESHAN, LIU LI
• Format: Patent
• Language: Chinese; English
• Published: 18.12.2013
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a tri-antibody sandwich ELISA (enzyme-linked immunosorbent assay) detection method of the IgG (Immunoglobulin G) of a tree shrew, which is applicable to the detection of the total content of IgG in the blood plasma of the tree shrew. The method comprises the following steps: coating an enzyme-label plate with the monoclonal antibody in the anti-rat IgG of the tree shrew as a capture antibody, and combining with the IgG of the tree shrew to be detected; and establishing the tri-antibody sandwich detection method of the IgG of the tree shrew by optimizing the concentration of the antibody and other multi-factor conditions by using the polyclonal antibody of the anti-rabbit IgG of the tree shrew as the secondary antibody and the commercial goat anti-rabbit IgG-HRP as the detection antibody. In the invention, the IgG level of the blood plasma of 30 tree shrews is detected by using the method, wherein according to the detection, the normal IgG level of the blood plasma of the tree shrews is