Dual affinity polypeptides for purification

The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contacting (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constan...

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Bibliographic Details
Main Author KYHSE-ANDERSEN JAN
Format Patent
LanguageChinese
English
Published 08.12.2010
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contacting (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide, [KD, t / KD, s ], is at least 10 DEG at standard conditions; and (b) recovering the target biomolecule by elution.
Bibliography:Application Number: CN200880124535