Analysis method for detecting cellulase activity of biomembranes

• Main Authors: GUO WENJIE, WAN JINQUAN, HUANG RONGZI, WANG YAN, MA YONGWEN
• Format: Patent
• Language: Chinese; English
• Published: 08.12.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses an analysis method for detecting the cellulase activity of biomembranes. The method specifically comprises the following steps of: (1) taking a proper quantity of cultured biomembranes, putting the biomembranes in a beaker, disrupting by using an ultrasonic cell disruption instrument,wherein the frequency is 20KC, the disrupting time is 5-10 minutes; and taking 100ml of bacterial suspension for measuring MLSS (Mixed Liquor Suspended Solids); (2) centrifuging the remained bacterial suspension, taking the supernate and adding a phosphate buffer and substrate microcrystalline cellulose for reacting for 30 minutes at constant temperature; (3) immediately adding a DNS (3,5-Dinitrosalicylic acid) reagent for terminating the reaction after the reaction ends, and putting the beaker in a boiling water bath for heating for 5 minutes; taking out for obtaining a constant volume, uniformly shaking, measuring the absorbance by using a spectrophotometer, and doing a blank experiment meanwhile; and (4