Method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances

• Main Authors: LI AIKE, LIU QIANRU, MA RONG, WANG YINGYAO, LUAN XIA
• Format: Patent
• Language: Chinese; English
• Published: 04.07.2012
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; DETERGENTS; ENZYMOLOGY; ESSENTIAL OILS; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFUMES; POLYSACCHARIDES; PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS; SPIRITS; STEROIDS; SUGARS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The invention discloses a method for extracting grease from oil-tea camellia seed kernel and recovering saponin and saccharide active substances, comprising the following steps of: (1) crushing and tempering: with hull-removed oil-tea camellia seed kernels as raw materials, crushing and grinding the materials, adding water for uniformly stirring, wherein the quality-volume ratio of the oil-tea camellia seed kernels to the water is 1kg:2-8L and controlling the temperature of the system to be 30-60DEG C; (2) carrying out the enzymolysis: adjusting the pH value of the system to be 3.5-8.0, adding pectinase the weight of which is 0.1-2 percent that of the camellia seed kernels and carrying out the enzymolysis for 2-5h; (3) heating and centrifuging: treating the system at the temperature of 80-90DEG C for 5-15min after enzymolysis; and (4) recovering the grease and the active substances: in which, the centrifuged and separated system comprises boiled oil, liquid phase and residue, taking out the boiled oil and spr