Separating method of icariside I monomer in Korean epimedium leaves

The invention provides a separating method of an icariside I monomer in Korean epimedium leaves, wherein the icariside I monomer is separated from the crude extract of the Korean epimedium leaves by using a high-speed counter current chromatography. The separating method comprises the following step...

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Bibliographic Details
Main Authors LIU ZHIQIANG, LIU ZHONGYING, DING YIDI
Format Patent
LanguageChinese
English
Published 24.11.2010
Subjects
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
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Summary:The invention provides a separating method of an icariside I monomer in Korean epimedium leaves, wherein the icariside I monomer is separated from the crude extract of the Korean epimedium leaves by using a high-speed counter current chromatography. The separating method comprises the following steps of: firstly selecting one phase in a pre-balanced two-phase solvent as a fixed phase, filling the fixed phase in a spiral tubular column, then enabling the spiral tubular column to rotate at a high speed at a certain rotating speed, and simultaneously pumping a mobile phase into the column at a certain flow speed. After the fluid dynamic balance of a system is achieved, a sample to be separated is injected into the system, and the components thereof are separated according to the different distribution coefficients of the components in the two phases. Through PLC (Programmable Logic Controller) detection, the purity of icariside I can reach more than 99 percent, and the method is simple and convenient and has hig
Bibliography:Application Number: CN20101206046