Preparation method of bacillus licheniformis raw powder with 100 billion live spores per gram

• Main Authors: YAN SHIJUN, ZHANG JINGMING, LIU HEMEI, XIE XIANG, CHEN YOUXIANG, YANG KEHUA, CHEN ZHENMIN, LIU HUAMEI, YU NA, HUANG ZHIYUAN, WANG QIAOMEI, XIE PAN, LI QING, LI KUN, CHENG ZHIGUO, GONG YONGHUA
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention discloses a preparation method of bacillus licheniformis raw powder with 100 billion live spores per gram. The method comprises the following steps of: (A) screening a fermentation culture medium of a strain BL KN-08, designing an experiment by adopting a response surface method invented by Box-Behken, and using a spore number as a screening index to obtain a culture medium formula beneficial to improving the spore number; (B) increasing the fermentation biomass, carrying out batch fermentation through the culture medium formula screened by using the response surface method to determine an optimal fermentation process beneficial to generating spores, and further increasing the spore number through fed-batch fermentation; and (C) extracting and recovering the spores. The bacillus licheniformis raw powder prepared by using the process has the advantages of clean and safe production, high recovery rate, stable product spore number, and the like, and the live spore number of the powder reaches 100 b