Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola

• Main Authors: WU LONGHUA, BEN AILING, TAN WEINA, LUO YONGMING, WANG SONGFENG
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2012
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a high-frequency plant regeneration technique for tissue culture of repairing plant Sedum plumbizincicola, which comprises the following steps: respectively taking the stem segment, the stem tip and the leaf of the repairing plant Sedum plumbizincicola for tissue culture and propagation, and respectively establishing high-frequency regeneration systems for the stem segment,the stem tip and the leaf. Since the stem segment, the stem tip and the leaf of the Sedum plumbizincicola are directly selected during propagation, the materials can be obtained easily, the scope of the materials for the propagation of the Sedum plumbizincicola is widened, the propagation method is rapid and high-efficiency, and conditions are provided for physiological biochemical researches andmolecular biological researches. Through respectively establishing the high-frequency plant regeneration systems for the stem segment, the stem tip or the leaf, the method is suitable for the screening and the micropropagation