Root tip detoxification and rapid propagation technology for purple potatoes

The invention discloses root tip detoxification and rapid propagation technology for purple potatoes and belongs to the technical field of plant detoxification and tissue culture rapid propagation. The method comprises the following steps of: (1) preparing a culture medium; (2) performing root tip d...

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Main Authors LIU JIAN, YU CHULANG, CHENG YE, YANG YONG, CHEN JIANPING, SHEN LAN, WANG FANG, WANG XUMING, YAN CHENGQI, ZHU HONGFEN, HUANG JIAN, XU GANG, YANG JIAN, JIANG YIHONG
Format Patent
LanguageChinese
English
Published 06.10.2010
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:The invention discloses root tip detoxification and rapid propagation technology for purple potatoes and belongs to the technical field of plant detoxification and tissue culture rapid propagation. The method comprises the following steps of: (1) preparing a culture medium; (2) performing root tip detoxification and rapid propagation; and (3) performing multi-reverse transcription-polymerase chain reaction (RT-PCR) synchronous and rapid detection on complex virus infection of a purple potato test tube plantlet or a test tube mini-tuber and the like. In the technology, each level of culture medium is adjusted according to the characteristic of the purple potato and conventional stem tip detoxification is changed into root tip detoxification so as to simplify operation process, avoid high pollution rate and improve working efficiency. Moreover, the technology has the advantages of good inoculant differentiation, high test tube plantlet propagation speed, complete detoxification, high mini-tuber growth vigor, mo
Bibliography:Application Number: CN20101212600