Bordetella parapertussis-containing whole-cell vaccine composition

• Main Authors: KOMATSU EIJI, WATANABE MINEO
• Format: Patent
• Language: Chinese; English
• Published: 03.12.2014
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

An objective of the present invention is to provide a whole-cell bacterial vaccine composition for preventing whooping cough caused by Bordetella parapertussis, comprising whole cells, whole-cell homogenate, or cell lysate of B . parapertussis as an immunogen, and methods for producing them. To accomplish the above-mentioned objective, first, the present inventors cultured B . parapertussis cells at 37°C for three days, adjusted the cell concentration to 10 10 cells/mL, and added a formaldehyde solution to a final formalin concentration of 0.2% for inactivation. These bacterial cells were adjusted to be equivalent to a cell concentration of 10 10 cells/mL by adding a sterilized physiological phosphate buffered saline to obtain a vaccine composition. The vaccine composition of the present invention was inoculated into mice (3.5 weeks old), and the mice were infected with a B . parapertussis suspension solution. The results revealed that the mice had a protective activity against B . parapertussis infection, an