Enzyme preparation using chlorphyllase as tobacco processing accessories, preparation method and application method

• Main Authors: CHEN YINGKUN, HAN WEI, HE BAOYU
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CIGARETTES; CIGARS; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SMOKERS' REQUISITES; SNUFF; SPIRITS; TESTING; TOBACCO; VINEGAR; WINE

The invention provides an enzyme preparation using chlorphyllase as tobacco processing accessories, a preparation method and an application method, which belong to an enzyme preparation added before the tobacco redrying. The invention uses treated later stage vertex-weighted waste materials in the tobacco planting process as extracting raw materials of the chlorphyllase, 250 U/mL to 450 U/mL chlorphyllase raw liquid or concentrated liquid is prepared in multiple batches, the activity of prolease is inhabited by a buffer solution with PMSF in the extraction process, and metal ions are removed by an EDTA complexing agent. The invention also provides a convenient chlorphyllase enzyme activity measuring method. Diluent of the invention is used for replacing secondary leaf wetting water duringsheet tobacco redrying, chlorophyll remained in cured tobacco can be converted, and the tobacco fragrance and the quality can be improved.