Construction of siRNA recombinants 191 by aiming at CDK2 genes and application

The invention discloses construction of RNAi recombinant plasmids pGPU6/GFP/Neo-siRNA191 by aiming at CDK2 genes and the effect of the RNAi recombinant plasmids pGPU6/GFP/Neo-siRNA191 on the inhabitation of the hepatoma carcinoma cell proliferation. An RNAi technical principle is utilized for design...

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Bibliographic Details
Main Authors LI HEWEI, YU SHUILAN, LI LING, LI NA, SONG GAOCHEN, YU YINGJUN, ZHAO YANG
Format Patent
LanguageChinese
English
Published 14.07.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses construction of RNAi recombinant plasmids pGPU6/GFP/Neo-siRNA191 by aiming at CDK2 genes and the effect of the RNAi recombinant plasmids pGPU6/GFP/Neo-siRNA191 on the inhabitation of the hepatoma carcinoma cell proliferation. An RNAi technical principle is utilized for designing siRNA191 using CDK2 as target genes, template DNA capable of transcribing shRNA is designed and synthesized, the template DNA is directionally cloned to eukaryotic expression vectors pGPU6/GFP/Neo, the digestion identification and DNA sequence checking technology is used for sieving, and the recombinant plasmids pGPU6/GFP/Neo-siRNA191 are successfully constructed. The recombinant plasmids can obviously inhabit the expression of the CDK2 genes in hepatoma carcinoma cells, and are used for preparing novel antitumor biological preparations and antitumor gene medicine.
Bibliography:Application Number: CN20081209713