Method for preparing bacterial ghost by combination of antibacterial peptide and ultrahigh pressure and application thereof

• Main Authors: SHENG XIANGPENG, DU CHONGTAO, HAN WENYU, SUN ZHANGJIANG, LEI LIANCHENG, JIANG LINA, XIE FANG
• Format: Patent
• Language: Chinese; English
• Published: 31.03.2010
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention discloses a method for preparing a bacterial ghost by the combination of an antibacterial peptide and ultrahigh pressure and application thereof, wherein the method comprises the following steps of: adding the antibacterial peptide when the bacterial strain is cultured until the logarithmic growth phase (the OD600 is 0.4 to 0.6) for action overnight at a temperature of 4 DEG C or for2 to 4 hours at the room temperature; centrifugally collecting the thalli and washing the thalli twice by normal saline, adding normal saline for re-suspension, and performing ultra-high pressure treatment at a pressure of between 200 and 250MPa; and centrifugally collecting the thalli and washing the thalli twice by normal saline, and finally obtaining the bacterial ghost. The invention providesa novel method for preparing the bacterial ghost, which has the advantages that: the effect is good, the crackling efficiency is as high as over 99.9999999 percent, the operation is simple, and the method can be widely used f