Supercritical fluid extract technology of ganoderma volatile oil, ganodenic acid and ganoderma polysaccharide

• Main Authors: YU TAOSHENG, XIA ZHILAN
• Format: Patent
• Language: Chinese; English
• Published: 20.07.2011
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; STEROIDS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; THEIR PREPARATION OR CHEMICAL WORKING-UP

The present invention discloses a supercritical fluid extract technology of ganoderma volatile oil, ganodenic acid and ganoderma polysaccharide, which comprises the following steps: (1) drying and crushing ganoderma mycelium or ganoderma sporocarp; (2) adding ganoderma microbe powder into the extraction cauldron of a supercritical CO2 fluid extracting machine, setting the extracting pressure to be 15-25 MPa and the temperature to be 38-42 DEG C, leading 95-105L/min CO2 fluid, extracting for 1.5-2.5h, and obtaining the ganoderma volatile oil form a separation kettle; and (3) adjusting the extracting pressure to be 28-32 MPa and the extracting temperature to be 44-47 DEG C, taking distilled water as the entrainer, extracting for 1.5-2.5h, obtaining the water solution containing the ganodenic acid and ganoderma polysaccharide, adding 3.5-4.5 times of volume of 95% (V/V) ethanol in the solution to generate flocculent precipitate, placing for 3-6h, centrifugalizing, separating, freezing and drying the precipitate,