Method for producing acteoside by fresh herba cistanches

• Main Authors: LIU XIN, PENG QINGYUN, ZHAO SHUYAN, HE XIUYAN, TIAN XIAOLING, JIA BAOGUO, CHEN YATING, LIU XIAOZHUO, XIAO JIAN
• Format: Patent
• Language: Chinese; English
• Published: 11.01.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SPIRITS; SUGARS; VINEGAR; WINE

The invention provides a method for producing acteoside by fresh herba cistanches, comprising the following steps: carrying out such system processes as enzyme deactivation at high temperature, cell disruption, size extraction and separation and purification on fresh herba cistanches fleshy stems to obtain herba cistanches extract, taking the obtained herba cistanches extract as substrates for catalytic reaction and beta-glucosidase, an industrial immobilized enzyme, as a biocatalyst to catalyze echinacoside in hydrolysis substrates to enable the ends of glucosyl ligands to be broken and the echinacoside to be directionally converted into high-activity monomeric compounds of the acteoside, and obtaining powder or dry products abundant in the acteoside by carrying out the processes of concentration and drying on the herba cistanches extract undergoing catalytic reaction, wherein, by adjusting the conditions of the separation and purification processes, the percentage content of phenylethanoid glycosides in the