Method for fast detecting beta-lactamase contained in milk product by optical fiber biosensor

• Main Authors: ZHAO CHUNCHENG, YANG TINGTING, ZHANG JIANZHONG, ZHAO XIAOLIAN, XU ZUQI, MA ZHENWAN, CAI JIANRONG
• Format: Patent
• Language: Chinese; English
• Published: 13.01.2010
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for fast detecting beta-lactamase contained in milk products by an optical fiber biosensor, belonging to the field of the optical fiber biosensor and comprising an optical system, an electronic system, a data collection system, a control and processing system, optical fibers and a sensor, wherein optical fiber material is polystyrene; the sensor is provided with a sensitive film, the sensitive film contains a beta-lactamase monoclonal antibody fixed by glutaraldehyde and marked by Cy5, and the monoclonal antibody is prepared by taking the beta-lactamase as an antigenic and immunized mouse, fusing cells, and the like. The invention has the advantages of high sensitivity, electromagnetic radiation resistance, electronic frequency interference resistance, corrosion resistance, small instrument volume, portability, few needed detection sample, rapid response time, easy operation, low cost, and the like and can be used for detecting the beta-lactamase contained in the milk products