Preparation method of raw powder of each gram of bacillus subtillis with five hundred billion of live germs
The invention discloses a preparation method of raw powder of each gram of bacillus subtillis with five hundred billion of live germs, which comprises the following steps: (A) the screening of a Bs strain: collecting a sample from soil and water; weighing the sample on a sterile and ultraclean table...
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Main Authors | , , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
06.01.2010
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a preparation method of raw powder of each gram of bacillus subtillis with five hundred billion of live germs, which comprises the following steps: (A) the screening of a Bs strain: collecting a sample from soil and water; weighing the sample on a sterile and ultraclean table and then loading the sample in a small triangular flask; adding sterile water into the triangular flask and standing still the sample; (B) the confirmation of the Bs strain: carrying out molecular classification according to a 16S rDNA sequence and together testing effective basic groups of the 16S rDNAs of the strain and identifying the strain; (C) a culture medium of the Bs: utilizing a single factor experiment to select a factor from numerous carbon sources, nitrogen sources and trace element factors firstly by adopting a response surface experiment designed by Box-Behken and using the germ number as a screening index; (D) using a high-density liquid feed-batch fermentation technology for improving the quantity |
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Bibliography: | Application Number: CN2009162964 |