Preparation method of raw powder of each gram of bacillus subtillis with five hundred billion of live germs

The invention discloses a preparation method of raw powder of each gram of bacillus subtillis with five hundred billion of live germs, which comprises the following steps: (A) the screening of a Bs strain: collecting a sample from soil and water; weighing the sample on a sterile and ultraclean table...

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Main Authors LIU HUAMEI, YU NA, WANG QIAOMEI, HUANG ZHIYUAN, MIAO HUAJUN, LI QING, XIE XIANG, YANG KEHUA, CHEN YOUXIANG, CHEN ZHENMIN, CHENG ZHIGUO
Format Patent
LanguageChinese
English
Published 06.01.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a preparation method of raw powder of each gram of bacillus subtillis with five hundred billion of live germs, which comprises the following steps: (A) the screening of a Bs strain: collecting a sample from soil and water; weighing the sample on a sterile and ultraclean table and then loading the sample in a small triangular flask; adding sterile water into the triangular flask and standing still the sample; (B) the confirmation of the Bs strain: carrying out molecular classification according to a 16S rDNA sequence and together testing effective basic groups of the 16S rDNAs of the strain and identifying the strain; (C) a culture medium of the Bs: utilizing a single factor experiment to select a factor from numerous carbon sources, nitrogen sources and trace element factors firstly by adopting a response surface experiment designed by Box-Behken and using the germ number as a screening index; (D) using a high-density liquid feed-batch fermentation technology for improving the quantity
Bibliography:Application Number: CN2009162964