Separation and purification method for improving specific activity of antihypertensive peptide

• Main Authors: HAN FUSEN, WU QIAOLING, HU YANXUE, LIU JIE, HAN HONGYUN, WANG YONGGANG, LI WEIQIANG
• Format: Patent
• Language: Chinese; English
• Published: 21.10.2009
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention discloses a separation and purification method for improving the specific activity of an antihypertensive peptide, which can realize the industrialization. The separation and purification method for improving the specific activity of the antihypertensive peptide is realized through the following technical scheme: the components of the antihypertensive peptide are prepared into 0.1-1mg/ml of aqueous solution for ultrafiltration, and the ultrafiltration is performed by adopting an ultrafiltration membrane under the pressure of 0.1-0.15 MPa until the volume of the ultrafiltrate reaches three fourths of that of the stock solution. The industrialized separation and purification of the antihypertensive peptide can be realized by the ultrafiltration method, the experimental results show that the activity of the raw material can be improved by ultrafiltration, and the specific activity of the raw material is greatly improved; and when the volume of the ultrafiltrate reaches three fourths of that of the