Method for extracting validamycin A raw powder from validamycin fermentation liquor

• Main Authors: LIU HUAMEI, HUANG ZHIYUAN, LI QING, XIE XIANG, YANG KEHUA, CHEN YOUXIANG, ZHENG QINWEI, CHENG ZHIGUO, CHEN ZHENMIN
• Format: Patent
• Language: Chinese; English
• Published: 26.10.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CHEMISTRY; DERIVATIVES THEREOF; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; SUGARS; TRANSPORTING; TRAPPING

The invention discloses a method for extracting validamycin A from validamycin fermentation liquor and refining the validamycin A. The method comprises the following steps: A. the validamycin fermentation liquor is directly handled by using a sawtooth ultrafiltration membrane system, the automatic control is adopted for the membrane system, and the output ultrafiltrate can directly enter in an ion exchange system after the validamycin fermentation liquor enters in the membrane system; B. cation exchange resin and anion exchange resin are selected and combined, and the foreign ions in the validamycin filtrate are removed; C. macroporous cation exchange resin is selected for absorbing the validamycin A, and the resin has good selectivity, large adsorbing capacity of the validamycin A, high mechanical strength and anti-pollution capability and long service life and is not easily broken; and D. due to the adoption of the PH gradient adsorption process, the PH value of the validamycin filtrate entering in the adso