Fast appraisal method for purity of Brassica napus L. hybrid seed

The invention discloses a method for approving the purity of cabbage type rape hybrid seed. The method comprises the steps as follows: the seed is squeezed and is soaked in 2-chlorohydrin solution for 2 to 3 hours for picking up prolamin; then electrophoretic separation is carried out by separation...

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Bibliographic Details
Main Authors CHEN WEIJIANG, QU LIANG, LI MEI, WANG TONGHUA, FAN LIANYI
Format Patent
LanguageChinese
English
Published 13.05.2009
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The invention discloses a method for approving the purity of cabbage type rape hybrid seed. The method comprises the steps as follows: the seed is squeezed and is soaked in 2-chlorohydrin solution for 2 to 3 hours for picking up prolamin; then electrophoretic separation is carried out by separation gel and stacking gel; the processes of fixing, dying, decoloring and banding are carried out after electrophoresis; and finally, the purity of the rape hybrid seed is calculated by tape reading. The approving method has the advantages of simple operation, low cost, high efficiency, high accuracy, etc.
Bibliography:Application Number: CN20081143770