Fermentative production of acetone from renewable resources by means of novel metabolic pathway

The invention describes a process for the preparation of acetone starting from acetyl-coenzyme A comprising the process steps A. enzymatic conversion of acetyl-CoA to give acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA, and C. decarboxylation of acetoacetate to...

Full description

Saved in:
Bibliographic Details
Main Authors ORSCHEL MATTHIAS, VERSECK STEFAN, SCHMIDT WILFRIED, BAHL HUBERT JOHANNES, SCHAFFER STEFFEN, SCHMIDT FRIEDRICH GEORG, FREITAG WERNER, GRUND GERDA, MAY ANTJE
Format Patent
LanguageChinese
English
Published 06.05.2009
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:The invention describes a process for the preparation of acetone starting from acetyl-coenzyme A comprising the process steps A. enzymatic conversion of acetyl-CoA to give acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA, and C. decarboxylation of acetoacetate to give acetone and CO2, which process is characterized in that, in process step B, the coenzyme A is not transferred to an acceptor molecule. Moreover, surprisingly, process step B is catalyzed by enzymes from the classes acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase. This is an entirely novel metabolic pathway since the enzymatic hydrolysis of acetoacetyl-CoA without the simultaneous transfer of CoA to a receptor molecule has never been described before for any microbial enzyme.
Bibliography:Application Number: CN20081173919