Process for producing fire resistant alpha-diastase
The invention discloses a preparation method of thermostable Alpha-amylase, pertaining to the technical field of bioengineering enzyme preparation. The preparation method uses bacillus licheniformis as strains and corns as carbon and nitrogen sources to produce the Alpha-amylase of high temperature...
Saved in:
Main Authors | , , |
---|---|
Format | Patent |
Language | Chinese English |
Published |
01.12.2010
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The invention discloses a preparation method of thermostable Alpha-amylase, pertaining to the technical field of bioengineering enzyme preparation. The preparation method uses bacillus licheniformis as strains and corns as carbon and nitrogen sources to produce the Alpha-amylase of high temperature resistance and high activity by fermenting in a first-grade seed tank, batch fermenting with feeding in big tank, fine filtering and sterilizing process. The fermentation capacity can reach a top of 13480u/ml, with an average level of enzymatic production with fermentation of 12360u/ml. The capacity is 54.5 percent higher than the fermenting level which is 7982u/ml before the prior art is optimized. Every standard ton of enzyme consumes 19.8kg of starch, being 5kg less than the batch fermentingwhich consumes 24.8kg, thus forming a complete set of fermenting feeding batch technique of thermostable Alpha-amylase and realizing the industrial scale production of thermostable Alpha-amylase. |
---|---|
Bibliography: | Application Number: CN20081141343 |