Process for producing fire resistant alpha-diastase

The invention discloses a preparation method of thermostable Alpha-amylase, pertaining to the technical field of bioengineering enzyme preparation. The preparation method uses bacillus licheniformis as strains and corns as carbon and nitrogen sources to produce the Alpha-amylase of high temperature...

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Bibliographic Details
Main Authors DU BAOAN, ZHANG XINWU, CUI YUEYUAN
Format Patent
LanguageChinese
English
Published 01.12.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a preparation method of thermostable Alpha-amylase, pertaining to the technical field of bioengineering enzyme preparation. The preparation method uses bacillus licheniformis as strains and corns as carbon and nitrogen sources to produce the Alpha-amylase of high temperature resistance and high activity by fermenting in a first-grade seed tank, batch fermenting with feeding in big tank, fine filtering and sterilizing process. The fermentation capacity can reach a top of 13480u/ml, with an average level of enzymatic production with fermentation of 12360u/ml. The capacity is 54.5 percent higher than the fermenting level which is 7982u/ml before the prior art is optimized. Every standard ton of enzyme consumes 19.8kg of starch, being 5kg less than the batch fermentingwhich consumes 24.8kg, thus forming a complete set of fermenting feeding batch technique of thermostable Alpha-amylase and realizing the industrial scale production of thermostable Alpha-amylase.
Bibliography:Application Number: CN20081141343