Mixed bacterial culture for atrazine degradation

• Main Authors: HRSAK DUBRAVKA, HAVRILUK MAJA
• Format: Patent
• Language: Chinese; English
• Published: 25.03.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS; CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING ORPROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FIRE-FIGHTING; HUMAN NECESSITIES; LIFE-SAVING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention provides a mixed bacterial culture, designated as the culture Atz Mix 1, which originates from the soil exposed to long-term contamination with atrazine and other s-triazine compounds. The culture Atz Mix 1 degrades atrazine at various tempetaures (1O DEG C to 30 DEG C) in a wide range of atrazine concentrations (several ppb to ten thousand ppm), without formation of toxic metabolites and leading to the complete atrazine degradation (mineralization). Atz Mix 1 is a stable mixed culture consisting of at least four members of different morphological, physiological and catabolic properties. Molecular characterization of the culture Atz Mix 1 showed the presence of catabolic genes trzN,atzB, atzC coding the enzymes for the degradation of atrazine to cyanuric acid and the gene trzD coding the enzymes for subsequent opening of s-triazine ring whereas high degree of mineralization of ring labelled [ C] atrazine (approximately 80%) confirmed that the degradation proceeded to yield CO2 and NH4.