Method for cleavage of peptide bond at C-terminus of peptide, and method for determination of C-terminal amino acid sequence of peptide

• Main Authors: MIYAZAKI KENJI, KAMIJO KEN ICHI, TUSGITA AKIRA
• Format: Patent
• Language: Chinese; English
• Published: 04.03.2009
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

Disclosed are: a method for cleaving a peptide bond at the C-terminus of a peptide, wherein the method is improved in the lowering of sensitivity and produces little side reactions; and a method for determination of the C-terminal amino acid sequence of a peptide. The method for cleaving a peptide bond at the C-terminus of a peptide comprises the steps of: reacting the peptide with a solution of a perhalogenated carboxylic acid containing an alcohol so as to esterify a glutamic acid residue in the peptide; and reacting the peptide with an anhydrous alkanoic acid so as to produce a C-terminal deficient peptide wherein the C-terminal amino acid residue in the peptide is deleted one by one. The method for determination of the C-terminal amino acid sequence of a peptide comprises the steps of: reacting the peptide with a solution of a perhalogenated carboxylic acid containing an alcohol so as to esterify a glutamic acid residue in the peptide; reacting the peptide with an anhydrous alkanoic acid so as to produce