Method for assisting lysozyme in vitro refolding by means of linear poly N-isopropyl acrylamide

The invention discloses a method adopting linear poly N-isopropyl acrylamide adhesive to assist the renaturation of muramidase in vitro, comprising the following steps that: 1) the linear N-isopropyl acrylamide is prepared by a free radical polymerization method; 2) the linear poly N-isopropyl acryl...

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Bibliographic Details
Main Authors GUAN YIXIN, YAO SHANJING, GE XIANG, CHEN YINGYING
Format Patent
LanguageChinese
English
Published 05.11.2008
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Summary:The invention discloses a method adopting linear poly N-isopropyl acrylamide adhesive to assist the renaturation of muramidase in vitro, comprising the following steps that: 1) the linear N-isopropyl acrylamide is prepared by a free radical polymerization method; 2) the linear poly N-isopropyl acrylamide is used to assist the renaturation of muramidase in vitro. The linear poly N-isopropyl acrylamide is taken as a novel protein in vitro additive which can increase the processing concentration of the renaturation muramidase, lower the production cost, and improve the activity recovery rate of the muramidase up to about 80 percent when the processing concentration is 600 Mug/mL; while the activity recovery rate of the dilution renaturation on the same condition is only 10 percent. The gel has the advantages of high efficiency, convenient operation and simple technique, etc., thereby having good potential application in the protein in vitro renaturation field.
Bibliography:Application Number: CN2008162428