Method for manufacturing high purified factor IX

The present invention relates to a method for manufacturing a highly purified human blood coagulation factor IX, including: subjecting objects containing human blood coagulation factor IX (derived from human plasma or recombinant cell culture medium) to anion exchange chromatography, cation exchange...

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Bibliographic Details
Main Authors LEE SUNG-RAE, CHOI YONG-WOON, SON KI-WHAN, KANG YONG, SUNG HARK-MO, HUH JAE-WOOK, KIM KI-YONG
Format Patent
LanguageChinese
English
Published 04.07.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a method for manufacturing a highly purified human blood coagulation factor IX, including: subjecting objects containing human blood coagulation factor IX (derived from human plasma or recombinant cell culture medium) to anion exchange chromatography, cation exchange chromatography, heparin affinity chromatography, preparing the human blood coagulation factor IX, and including: subjecting the S/D treatment (Solvent/Detergent treatment) to inactive viruses or removing the viruses through nanofiltration, thereby obtaining a highly purified, safe coagulation factor IX having a specific activity of above 150 IU/mg, with substantially inactivation or removal of all impure proteins and viruses.
Bibliography:Application Number: CN2006839024