Method for breeding transgenic crop with iron/zinc and nicotinamide rich grains and uses thereof

• Main Authors: ZHENG YE, CHENG LONGJUN, LEI XINGEN, BEI XIAOSHU, SHOU HUIXIA, WU PING, ZHENG LUQING
• Format: Patent
• Language: Chinese; English
• Published: 18.06.2008
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The present invention discloses a cultivation method of a transgenic crop, the grain of which is rich in iron or zinc and nicotinamide, comprising the steps as follows: firstly, nicotinamide synthetase gene is selected, and a prompter and a terminator of endosperm specific expression gene are selected; secondly, the construction of an transgenic vector of endosperm specific expression is that the prompter of the endosperm specific expression gene, the nicotinamide synthetase gene and the terminator used for commonly used plant expression are in forward series connection to obtain a gene expression cassette that crop endosperm expressively expresses NAS gene and accordingly to obtain the transgenic vector; thirdly, transgenic gene crop is cultivated. The method is adopted, which can improve the content of iron and zinc and the content of NA in the grains of the crop and accordingly the crop containing high biological effective iron element is obtained. The present invention also discloses the purpose of the tr